Postdoc in Proteomics and Bioinformatics
Novo Nordisk Foundation Center for Basic Metabolic Research
Faculty of Health and Medical Sciences
University of Copenhagen
The University of Copenhagen is seeking a highly motivated and talented postdoctoral fellow to commence October 1, 2021 at the Novo Nordisk Foundation Center for Basic Metabolic Research (CBMR), University of Copenhagen.
The postdoctoral fellowship is part of the Novo Nordisk Foundation Center for Basic Metabolic Research International Postdoctoral Program www.cbmr.ku.dk/postdocprogram.
The Novo Nordisk Foundation Center for Basic Metabolic Research’s vision is to strengthen interdisciplinary research that transforms our basic understanding of the mechanisms that underlie metabolic health and disease, and to accelerate this knowledge toward new prevention and treatment strategies. We were established in 2010 and in 2017, we moved into new laboratories, offices and meeting rooms in the Maersk Tower www.maersktower.ku.dk.
The Center has around 240 employees who work in an international, highly collaborative research environment across a range of biomedical disciplines. You can read more about us here www.cbmr.ku.dk.
The current position is in the Program for Integrative Physiology and Environmental Influences. The candidate will be part of the Functional Proteomics in Metabolism Group headed by Associate Professor Atul Deshmukh and work in close collaboration with the Novo Nordisk Foundation Center for Protein Research at the University of Copenhagen. The successful candidate will work to integrate proteomics and functional physiology, to answer basic and applied research questions related to physical exercise, diet and metabolism. He or she will be embedded within the Center and work in a scientific environment that bridges several disciplines of proteomics and metabolic diseases. Our facilities include state-of-the-art laboratories and core facilities and the position provides unique opportunities to advance expertise in state-of-the-art quantitative mass spectrometry and data analysis of large datasets. Striving for academic excellence, collegial respect and freedom tempered by responsibility, we offer creative, friendly and stimulating working conditions in a dynamic and strong scientific environment with the opportunity to plan work independently to further professional and personal development.
Skeletal muscle is the largest tissue in the human body and plays an important role in locomotion and whole body metabolism. It accounts for ~80% of insulin stimulated glucose disposal. Skeletal muscle insulin resistance, a primary feature of Type 2 diabetes, is a consequence of defects in intracellular signalling leading to failure in insulin-mediated effects on glucose and lipid metabolism. Insulin signalling diverges into multiple branches affecting every organelle of the cell, and for a subset of proteins, this involves translocation from one compartment to another (e.g. FOXO family proteins). This illustrates that insulin signalling is rather complex and dynamic. Identifying these dynamics in skeletal muscle will reveal novel insight into insulin signalling and how muscle organelles respond in health and disease.
The overarching aim of this research project is to identify and understand subcellular organelle level protein dynamics of insulin stimulated skeletal muscle. We hypothesize that insulin regulates skeletal muscle metabolism through compartmentalization of proteins and phosphoproteins. Your task will be to apply mass spectrometry-based proteomics to map insulin-induced changes in organellar proteome and phosphoproteome in skeletal muscle from control and diet-induced insulin-resistant mice. The results from the proteomics analysis will be validated by high-resolution microscopy in collaboration with the microscopy facility at the University of Copenhagen. You are expected to 1) perform organelle fractionation on skeletal muscle tissue 2) prepare samples for proteomics and phosphoproteomics analysis 3) perform advanced bioinformatics analysis to study organelle proteomics and phosphoproteomics 4) perform high-resolution microscopy in collaboration with the core facility. For the relevant literature and technical details, please refer to these articles PMIDs: 16615899, 30352176, and 27278775.
The fellowship is aimed at early career researchers with a basic science background or clinicians who aspire to a career in academic medicine. We are particularly interested in candidates who are familiar with integrative research approaches within the broad area of basic metabolic research with an application towards human pathophysiology.
- PhD in Biology or related sciences
- Excellent scientific track record in relation to career stage
- Knowledge concerning sample preparation for proteomics and phosphoproteomics analysis
- Knowledge concerning proteomics data analysis using Python, R or other programming languages
- Ability to analyse raw MS files using MaxQuant, Spectronaut, Skyline or similar platforms
- Willingness to implement new relevant methods for proteomics analysis
- Excellent English communication skills, both written and oral
- Hands-on experience using liquid chromatography and tandem mass spectrometry on Thermo Scientific instruments with standard software instrument control
- Experience with targeted proteomics (SRM, MRM)
- Experience with isobaric labelling (preferentially TMT)
- Certified with FELASA laboratory animal science
- Candidate with additional background in metabolism, diabetes and the measurement of proteins and posttranslational modifications within skeletal muscle would be highly regarded
The CBMR International Postdoctoral Program is open for applicants that hold a PhD degree awarded from a university outside of Denmark. The PhD degree has to be obtained before October 1, 2021. The program is also open for applicants with a Danish PhD degree who can document at least 12 months full-time research experience from outside Denmark. You are not eligible to apply for the program if you have been employed in a postdoctoral position for more than one year at the University of Copenhagen prior to the commencement of the fellowship.
Terms of employment
The employment as postdoc is a full time position for 3 years. Starting date is October 1, 2021.
Salary, pension and terms of employment will be in accordance with the agreement between the Danish Ministry of Finance and AC (Danish Confederation of Professional Associations). Depending on qualifications, a supplement may be negotiated.
Non-Danish and Danish applicants may be eligible for tax reductions, if they hold a PhD degree and have not lived in Denmark the last 10 years.
For further information about the position, please contact Associate Professor Atul Deshmukh at firstname.lastname@example.org. Questions regarding the CBMR International Postdoctoral Program must be directed to email@example.com. For questions regarding the recruitment procedure, please contact HR at firstname.lastname@example.org.
The University of Copenhagen International Staff Mobility office offers support and assistance to all international researchers on all issues related to moving to and settling in Denmark. Read more at www.ism.ku.dk.
Your online application must be submitted in English via the ‘Apply now’ link below. Furthermore, your application must include the following documents/attachments – all in PDF format:
- Cover letter expressing the motivation and previous research experience of the applicant (max 1 page)
- Curriculum vitae
- Copy of the PhD degree certificate and the Master degree certificate. In case the PhD has not yet been completed, a written statement from the supervisor confirming it will be obtained before October 1, 2021 is acceptable.
- List of publications
- References (name and contact details of at least two references)
Application deadline: April 19, 2021, 23.59pm CET
We reserve the right not to consider material received after the deadline, and not to consider applications that do not live up to the abovementioned requirements.
The further process
After the expiry of the deadline for applications, the authorized recruitment manager selects applicants for assessment on the advice of the hiring committee. All applicants are then immediately notified whether their application has been passed for assessment by an unbiased assessor. Once the assessment work has been completed, each applicant has the opportunity to comment on the part of the assessment that relates to the applicant him/herself.
You can read about the recruitment process at www.employment.ku.dk/faculty/recruitment-process.
The applicant will be assessed according to the Ministerial Order no. 242 of March 13, 2012 on the Appointment of Academic Staff at Universities.
The University of Copenhagen wishes to reflect the diversity of society and welcomes applications from all qualified candidates regardless of their personal background.
Part of the International Alliance of Research Universities (IARU), and among Europe’s top-ranking universities, the University of Copenhagen promotes research and teaching of the highest international standard. Rich in tradition and modern in outlook, the University gives students and staff the opportunity to cultivate their talent in an ambitious and informal environment. An effective organisation – with good working conditions and a collaborative work culture – creates the ideal framework for a successful academic career.