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Laboratory Technician for Cryo-electron Microscopy

Laboratory Technician for Cryo-electron Microscopy Group 
 
Novo Nordisk Foundation Center for Protein Research (www.cpr.ku.dk) at the Faculty of Health and Medical Sciences, University of Copenhagen, performs basic and applied discovery research on human proteins of biomedical relevance. The Center comprises a wide range of expertise and resources, from in silico target identification, mass spectrometry based proteomics, structural biology, protein production and characterization to elucidation of various disease mechanisms.  
 
The Taylor group (starting February 2018) is studying the structure and mechanism of biological molecular machines using cryo-electron microscopy. We are especially interested in resolving structural and functional pathways of bacteriophages (bacterial viruses) and human membrane proteins. 
 
Job description   
  
  • perform laboratory work within the areas of cloning, cell culture, recombinant expression, and protein purification of soluble and membrane proteins and complexes. Techniques used will include Gibson assembly cloning, sequence- and ligation-independent cloning and other cloning methods, CRISPR/Cas9-based bacterial genome modification, protein purification (including affinity purification, ion exchange, size exclusion chromatography), gradient-based ultracentrifugation, reconstitution of proteins into nanodiscs and proteolipsomes, bacteriophage production
  • evaluate and report experimental results and ensuring that research data and protocols are entered into an (electronic) lab notebook
  • maintain stocks, reagents, media and laboratory equipment  
  • share responsibility within the team in terms of defining objectives to meet deadlines 
  • participate in the general operation and maintenance of the laboratory facilities
  • carry out any other relevant duties as may reasonably be associated with the position and which may be required from time to time  
 
Experience and skills   
 
  • Laboratory technician degree  
  • Solid and documented work experience from high quality academic and/or industrial laboratories is required   
  • Proven experience in molecular biology and cell culture laboratory work   
  • Experience in protein purification and characterization  Computer proficiency (Word, Excel)   Experience with research database systems would be considered an asset
  • Good communication skills, both oral and written. Considering that the Center is a highly international research environment, good knowledge of English is required  
  • Integrity, reliability, enthusiasm, motivation, flexibility, confidence, capability to learn new methods and good collaboration skills
  • The ability to efficiently work as part of a team is essential   
  • Experience with membrane protein purification, nanodisc and/or liposome reconstitution would be beneficial but is not requirement
  • Experience with bacteriophages as well as experience with negative stain and/or cryo-electron microscopy sample preparation would also be beneficial
 
Terms of salary and employment   
 
Employment will be in accordance with the provisions of the collective agreement between the Danish Government and HK-STAT (Danish Technician Association). The position will be at the level of salary group 4 with the possibility to negotiate a salary supplement. To this is added a monthly contribution to a pension fund according to the collective agreement.  
 
The position is time-limited for 1 year in the first instance, with the possibility of a permanent position afterwards. Working hours are 37 hours per week.   
 
Start date: 1 March 2018 
 
To apply   
In order to be taken into consideration for this position, you must fill in an online application form which you will find on Copenhagen University's recruitment web page. We only accept applications sent through this link: www.employment.ku.dk   
 
The application must include:   
 
  • motivation letter 
  • curriculum vitae (including contact details of three references) 
  • copy of relevant diplomas   
 
Deadline for the application: 21 January 2018   
 
The University of Copenhagen wishes to reflect the diversity of society and encourage all qualified candidates to apply regardless of personal background. For further information regarding the position please contact: www.jobs@cpr.ku.dk   
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Part of the International Alliance of Research Universities (IARU), and among Europe’s top-ranking universities, the University of Copenhagen promotes research and teaching of the highest international standard. Rich in tradition and modern in outlook, the University gives students and staff the opportunity to cultivate their talent in an ambitious and informal environment. An effective organisation – with good working conditions and a collaborative work culture – creates the ideal framework for a successful academic career.

Contact

Lotta Avesson

Info

Application deadline: 21-01-2018
Employment start: 01-03-2018
Working hours: Full time
Department/Location: Novo Nordisk Foundation Center for Protein Research

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